食品与发酵工业2012，Vol.38Issue(6)：43-47,5.
ATP合成关键酶基因APT1在雅致放射毛霉中的克隆表达
Cloning and Experssion of Key Enzyme Gene APT1 for ATP Synthesis in Actinomucor elegans
朱家荣 1杨善岩 1陈丽芬 1杨光辉1
作者信息
- 1. 浙江工业大学生物与环境工程学院,浙江杭州310014
- 折叠
摘要
Abstract
To construct the engineering strain with genetic stability which can efficient to synthesize ATP,the key enzyme gene APT1 for ATP synthesis which from Saccharomyces cerevisiae was cloned by PCR.The sequence was cloned to corresponding site of pCB1004-Pgpd.Then the super expression plasmid pCB1004-Pgpd-APT1 which controlled by strong promoter Pgpd was obtained.PEG and CaCl2 mediated protoplast transformation of Actinomucor elegans with super expression plasmid was performed and ATP-overproducing transformants were obtained.The ATP yield and Moore conversion were increased 44.04% in comparison with those from original strain.关键词
APT1/雅致放射毛霉/酿酒酵母Key words
APT1/Actinomucor elegans/Saccharomyces cerevisiae分类
轻工纺织引用本文复制引用
朱家荣,杨善岩,陈丽芬,杨光辉..ATP合成关键酶基因APT1在雅致放射毛霉中的克隆表达[J].食品与发酵工业,2012,38(6):43-47,5.
