食品与发酵工业2012，Vol.38Issue(8)：47-52,6.
传统香肠中产生物胺肠细菌和乳酸菌分离方法的研究
Screening Procedure for Biogenic Amine-producing Lactic Acid Bacteria and Enterobacteria in Traditional Chinese Sausage
摘要
Abstract
A new method of monolayer cultivation coupled with double-layer color development was established. 96 strains of biogenic amines-producing lactic acid bacteria and 58 strains of biogenic amines-producing Enterobacteria were obtained. The 154 strains of biogenic amines-producing bacteria belonged to 5 species according to the results of polymerase chain reaction denaturing gradient gel eleetrophoresis （PCR-DGGE） analysis. After sequencing of PCR- products of 16S rDNA fragments and alignment in Genebank, the 5 strains of biogenic amines-producing bacteria were found to be Enterococcus faecium, Enterococcus faecalis, Enterobacter cloacae, Escherichia coli, and Enterobacter aero- genes. The presence of Biogenic amines-producing gene was demonstrated by special primers.关键词
生物胺/肠细菌/乳酸菌/分离鉴定Key words
biogenic amines/Enterobacteria/lactic acid bacteria/isolation and identification分类
轻工纺织引用本文复制引用
卢士玲,李开雄,徐幸莲,李蕊婷,马宇霞,李宝坤..传统香肠中产生物胺肠细菌和乳酸菌分离方法的研究[J].食品与发酵工业,2012,38(8):47-52,6.基金项目
国家自然科学基金项目：新疆熏马肠生物胺产生和累积机理研究 （）
石河子大学自然科学重点项目：新疆熏马肠中生物胺累积机理研究 （）
