食品与发酵工业2012，Vol.38Issue(8)：157-162,6.
乳品生产链金黄色葡萄球菌的污染、耐药性分析及β-内酰胺类药物耐药基因的检测
Analysis on Contamination and Drug Resistance and Detection of Beta-lactam Drug Resistance Genes in Staphylococcus aureus Isolated from the Dairy Production Chain
摘要
Abstract
To analyze the contamination situation and drug resistance of Staphylococcus aureus （Sa） in the dairy production chain, 523 samples of dairy food （raw milk, semi-finished milk, milk products） were collected. With ref- erence to GB 4789.10 -2010 and PCR, the isolation and identification of Sa were carried out using broth dilution method to determine the antimicrobial susceptibility to 12 antibiotics of Sa isolates. Multiplex PCR was used to analyze the relationship between the resistance to beta-lactams and the antimicrobial resistance genes （mecA, blaZ）. The Re- suits indicated that the total detectable rate of Sa was 24.9% （129 samples） , in which, the positive rate in raw milk, semi-finished milk and milk products were 37.5% , 7.1% and 0.0% respectively. All the isolates were mostly resist- ant to penicillin （ 97.7% ） , followed by resistance to ampicillin （ 95.4% ） , trlmethoprim/sulfamethoxazole （61.9%） , amoxicillin/clavulanic acid （61.7%） , erythromycin （39.7%） , tetracycline （36.6%） , clindamycin （35.2%）. All strains were susceptible to oxacillin, ceftiofur, florfenicol. 69.8% of the isolates were multi-drug resistant, the whole test strains were multi-drug resistant mainly to penicillins, sulfonamides, macrocyclie lipids, lincosamldes and tetracyclines. The positive rates for detection of nuc and blaZ genes by PCR were 100.0% , 60.5% and 0.0% respectively. There were a lot Correspondence between the resistance to beta-lactams and the detection rate of the resistance gene （blaZ） about Sa from different segments of dairy production chain. The contamination and drug resistance of Sa isolates recovered from dairy production chain were not optimistic, so we must take it seriously and avoid the occurrence of food-borne illness关键词
金黄色葡萄球菌/乳品生产链/污染/耐药性/多重PCRKey words
Staphylococcus aureus/dairy production chain/contamination/drug resistance/multiplex PCR分类
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凡琴,刘书亮,韩新锋,侯小刚,颜其贵..乳品生产链金黄色葡萄球菌的污染、耐药性分析及β-内酰胺类药物耐药基因的检测[J].食品与发酵工业,2012,38(8):157-162,6.基金项目
公益性行业（农业）科研专项子课题（200903055）资助 （）
