食品与发酵工业2012，Vol.38Issue(9)：94-98,5.
黄精多糖肽的单糖组成和抗氧化活性
The Monosaccharide Composition and Antioxidant Activity of Polygonatum Glycoprotein
摘要
Abstract
Polygonatum glycoprotein was extracted from polygonatum by hot water and sinking in alcohol. The tri- chloroacetic acid, the dialysis and the anion-exchange column chromatography were used to purify the Polygonatum glycoprotein. Monosaccharide composition of polygonatum glyeoprotein was studied by GC-MS and the antioxidant ac- tivity of Polygonatum glycoprotein were studied by Fenton oxidation, phosphomolybdenum complex method and pyro- gallol autoxidation method. The results showed that : The content of Polygonatum glycoprotein was 0.34% , the ratio of polysaccharide and protein was 5.4: 1, the monosaccharide composition of Polygonatum glyeoprotein mainly were rhamnose, arabinose, D-xylose, galactose, glucose and mannose polysugar and their relative percentage contents were: 2.12% , 1.81% , 2.51% , 65.4% , 14.3% , 13.8%. Scavenging ratio of hydroxyl radical, scavenging ratio of superoxide anion radical and total antioxidant activity of polygonatum glycoprotein increased gradually by the con- tents of polygonatum glycoprotein. Scavenging ability of hydroxyl radical and superoxide anion radical were higher than that of Vc, however the total antioxidant activity was lower than that of Vc.关键词
黄精/多糖肽/GC-MS/抗氧化活性Key words
polygonatum/glycoprotein/GC-MS/antioxidant activity分类
轻工纺织引用本文复制引用
梁引库,吴三桥,徐仲阳,李新生,杨海涛,刘军海..黄精多糖肽的单糖组成和抗氧化活性[J].食品与发酵工业,2012,38(9):94-98,5.基金项目
陕西省发改委陕南突破发展项目 （）
