食品与发酵工业2012，Vol.38Issue(9)：170-173,4.
毛细管气相色谱内标法同时测定奶粉中亚麻酸、ARA、EPA和DHA
Simultaneous Determination of Linolenic Acid, ARA, EPA and DHA in Milk Powder with Capillary GC by Internal Standard Method
黄杰 1余孔捷 1钱疆 1王丹红 1蔡姗姗 1薛芝敏1
作者信息
- 1. 福建出入境检验检疫局,福建福州350001
- 折叠
摘要
Abstract
A method using capillary gas chromatography for simultaneous determination of linolenic acid, ARA, EPA and DHA in milk powder was developed and heneicosanoic acid methyl ester was selected as the internal stand- ard substance. The samples were firstly saponified and methylated with KOH-CH3OH. These methyl esters were then analyzed with GC by Internal standard method. The linolenic acid, ARA, EPA, DHA and internal standard substance were separated from each other. There were good linear relationships between their concentration and their peak area, and the correlation coefficient were more than 0. 998. The recoveries for adding standards were 95.4% - 104.7% , and the coefficient of variation were 2. 168% - 4. 368%. The limits of detection were 1.0mg/100g. The method is simple, fast and accurate for the determination of linolenic acid, ARA, EPA and DHA in a lot of milk powder sam- pies.关键词
气相色谱内标法/奶粉/亚麻酸/ARA/EPA/DHAKey words
capillary GC/internal standard/milk powder/linolenic acid/ARA/. EPA/DHA分类
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黄杰,余孔捷,钱疆,王丹红,蔡姗姗,薛芝敏..毛细管气相色谱内标法同时测定奶粉中亚麻酸、ARA、EPA和DHA[J].食品与发酵工业,2012,38(9):170-173,4.
