食品与发酵工业2012，Vol.38Issue(9)：191-195,5.
茶多糖的分离纯化及其抗凝血活性
Study on Purification and Anticoagulant Activity of Tea Polysaccharides from Camellia Sinensis
摘要
Abstract
To study different components of tea polysaccharides and their anticoagulant activity,the tea polysac- charides were extracted by water and precipitated by alcohol, and purified by ion exchange chromatograph on DEAE sepharose CL-6B column, and its molecular weight was determined by high performance gel permeation chromato- graph. The anticoagulant activity of polysaccharide was also studied. The results showed polyamide method was effec- tive on deeolorization and deproteination, the decolorization and deproteination and polysaeeharides retention rate were 75.5% , 91.2% and 79.2% respectively; four eomponets were obtained after purification: TPS-1, TPS-2, TPS-3 and TPS-4. HPGPC method showed that the TPS-1, TPS-2 and TPS-3 were homogeneous polysaccharides. Their mo- lecular weight were 20760,24230 and 250643, separately, they are homogeneous. TPS-4 has two components of mo- lecular weight of 689113 and 4150, and it was heterogeneous. Anticoagulant activities in vitro tests showed TPS-4 could prolong activated partial thromboplastin time (ApTT) and thrombin time (TT). The result indicated TPS in the regulation of coagulation initiated via the intrinsic pathway.关键词
茶多糖/抗凝血/离子交换/高效凝胶渗透色谱/分子量Key words
tea polysaccharides/anticoagulant activity/ion exchange/high performance gel permeation chromatog- raphy/molecular weight分类
轻工纺织引用本文复制引用
谢亮亮,蔡为荣,张虹,陈勇..茶多糖的分离纯化及其抗凝血活性[J].食品与发酵工业,2012,38(9):191-195,5.基金项目
国家自然科学基金项目 （）
安徽省国际合作计划项目 （）
