食品与机械2012，Vol.28Issue(5)：79-82,4.DOI:10.3969/j.issn.1003-5788.2012.05.020
LAMP实时浊度法检测转基因水稻Bt63品系
Development of a real-time turbidimeter-based loop-mediated isothermal amplification method for rapid detection of transgenic rice Bt63
摘要
Abstract
Based on the loop-mediated isothermal amplification (LAMP) technology, four specific primers were designed according to the six regions of unique and specific integration junction sequences between the host plant genome DNA and the integrated gene of Bt63. Real-time monitoring of the LAMP reaction was achieved by a turbidimeter. The reaction temperature, sensitivity, specificity, stability and capability of testing sample were detected. The results showed that the method can specifically detect rice Bt63. The sensitivity of LAMP detection was 0.01%, which was 10 times more sensitive than the conventional PCR.关键词
环介导等温扩增技术/转基因水稻/实时浊度法/外源基因/内源基因/检测Key words
loop-mediated isothermal amplification/ transgenic rice/ real-time turbidimeter method/ exogenous gene/ endogenous gene/ detection引用本文复制引用
吴少云,唐大运,李琳,石磊..LAMP实时浊度法检测转基因水稻Bt63品系[J].食品与机械,2012,28(5):79-82,4.基金项目
广东省自然科学基金项目(编号:10451064101005159) （编号:10451064101005159）
广州市科技计划项目(编号:11C12080718) （编号:11C12080718）
厦门市重大科技计划项目(编号:3502Z20110001) （编号:3502Z20110001）
