中国食品学报2012，Vol.12Issue(8)：47-52,6.
高速逆流色谱法分离制备柿子中儿茶素及表儿茶素
Separation of Catechin and Epicatechin from Persimmon by High Speed Counter Current Chromatography
摘要
Abstract
HP2MGL macroporous resin was adopted to separate catechin and epicatechin, and then High-speed Counter Current Chromatography instrument was used for further separation and purification. The separation conditions were 800 r/min rotated rate, 1 mL/min flow rate in the solvent system was composed of normal butanol-ethyl acetate-wa-ter(1:2:3,V:V:V); the upper was used as the stationary phase and the lower phase was used as the mobile phase. The results indicated that 7.25 mg catechin and 0.43 mg epicatechin could be obtained with 58 mg persimmon extraction by HSCCC at the above conditions. The purity of catechin and epicatechin was individually 98.3% and 97.9% by High Performance Liquid Chromatographic analysis.关键词
高速逆流色谱/柿子/儿茶素/表儿茶素Key words
Counter-current chromatography/ persimmon/ catechin/ epicatechin引用本文复制引用
郭琳博,李宇华,周婧,岳溪,陈湘宁..高速逆流色谱法分离制备柿子中儿茶素及表儿茶素[J].中国食品学报,2012,12(8):47-52,6.基金项目
北京市中青年骨干项目(PHR201008416) （PHR201008416）
北京市优秀人才培养资助项目(2010D005021000002) （2010D005021000002）
农产品加工及贮藏工程北京市重点建设学科(PXM2009_014207_078172) （PXM2009_014207_078172）
