茶叶科学2012，Vol.32Issue(5)：411-418,8.
茶树类黄酮3-O-葡萄糖基转移酶基因的克隆和表达分析
The Gene Cloning and Expression Analysis of UFGT in Tea Plant [Camellia sinensis (L.) O.Kuntze]
摘要
Abstract
A glucosyltransferase gene UDP-flavonoid 3-O-glucosyl transferase was isolated from tea plant [Camellia sinensis (L.) O. Kuntze] and named CsUFGT. CsUFGT has 1 526 bp full length with open reading frame of 1380 bp which encodes 459 amino acids. The corresponding protein CsUFGT, with predicted molecular mass 49.486 kDa and predicted isoelectric point 5.96, shares 59% identity and 75% similarity with UFGT(P51094.2) in Vitis vinifer. CsUFGT includes a PSPG signal motif of typical plant glucosyltransferase. qRT-PCR analysis showed that the gene expressed in all tissues of tea plant [Camellia sinensis (L.) O. Kuntze], and had high expression in the fourth leaf and low expression level in root and stem.关键词
茶树/类黄酮3-O-葡萄糖基转移酶/基因克隆/表达分析Key words
Camellia sinensis, flavonoid 3-O-glucosyl transferase, gene cloning, expression analysis分类
农业科技引用本文复制引用
王晓帆,田艳维,王云生,高丽萍,夏涛..茶树类黄酮3-O-葡萄糖基转移酶基因的克隆和表达分析[J].茶叶科学,2012,32(5):411-418,8.基金项目
国家自然科学基金(30972401,31170647和31170282) （30972401,31170647和31170282）
