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植物乳杆菌ZS2058生物转化共轭亚油酸反应条件的优化研究

陈海琴 杨波 许庆炎 宋元达 陈卫 张灏

食品工业科技2012,Vol.33Issue(22):192-197,6.
食品工业科技2012,Vol.33Issue(22):192-197,6.

植物乳杆菌ZS2058生物转化共轭亚油酸反应条件的优化研究

Study on condition optimization of conjugated linoleic acid bioconversion by Lactobacillus plantarum ZS2058

陈海琴 1杨波 1许庆炎 1宋元达 1陈卫 1张灏1

作者信息

  • 1. 江南大学食品学院,食品科学与技术国家重点实验室,江苏无锡214122
  • 折叠

摘要

Abstract

The converted condition of LA to CLA by using Lactobacillus plantarum ZS2058,which was screened from the Chinese traditional fermented vegetable,was studied.According to the production of CLA,bioconversion of CLA by resting cells of L.plantarum ZS2058 in 1mL potassium phosphate buffer system was optimized.Through the single factor experiment and response surface analysis,374μg/mL of c9,t11-CLA was obtained under the reaction condition as follows:0.8mg/mL LA solution,4×1010cfu/mL cell mass,optimal temperature 37℃ and the optimal pH value 6.7,and the conversion ratio of c9,t11-CLA was 46.78%,which was valuable for producing CLA efficiently and studying its physiological function.

关键词

共轭亚油酸/生物转化/植物乳杆菌/转化率

Key words

conjugated linoleic acid/bioconversion/Lactobacillus plantarum/conversion ratio

分类

生物科学

引用本文复制引用

陈海琴,杨波,许庆炎,宋元达,陈卫,张灏..植物乳杆菌ZS2058生物转化共轭亚油酸反应条件的优化研究[J].食品工业科技,2012,33(22):192-197,6.

基金项目

“十一五”国家“863计划” ()

“十二五”国家“863计划” ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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