食品工业科技2012，Vol.33Issue(22)：213-217,5.
重组过氧化物酶A4-Prx酶学特性及其降解DDGS中ZEN的研究
Study on characterization of a recombinant peroxidase A4-Prx and its ZEN detoxification in DDGS
摘要
Abstract
The enzymatic characteristics of the recombinant peroxidase Acinetobacter sp.SM04(A4-Prx) was studied.Firstly,the effect of pH,temperature and H2O2 concentration on peroxidase A4-Prx activity were studied.Then,the orthogonal experiment was designed to receive the optimal ZEN(zearalenone) degradation rate of A4-Prx in DDGS(dried distillers' grains and soluble).The A4-Prx characterization result showed that it was an alkaline and protoheme independent peroxidase,and the optimal pH value,temperature and H2O2 concentration for peroxidase A4-Prx activity were pH8.5,75℃ and 25mmol/L respectively.And the optimum condition for ZEN decontamination were as follows:40mmol/L H2O2,9h,70℃ and 1∶2(solid-liquid ratio,g/mL).Under the optimum condition,ZEN degradation rate was 99.2%±0.2%.关键词
过氧化物酶/酶学特性/ZEN/DDGS/生物降解/正交实验Key words
peroxidase/enzymatic characteristics/zearalenone/DDGS/biodetoxification/orthogonal experiment分类
轻工纺织引用本文复制引用
吴晖,肖俊梅,陈艺,余元善,陈深亮,唐语谦..重组过氧化物酶A4-Prx酶学特性及其降解DDGS中ZEN的研究[J].食品工业科技,2012,33(22):213-217,5.基金项目
广东省大学生创新实验项目 （）
华南理工大百步梯攀登计划研究项目 （）
华南理工大学2012“学生研究计划” （）
国家自然科学基金项目 （）
