食品工业科技2012，Vol.33Issue(22)：292-295,302,5.
野生牛肝菌多糖提取工艺的优化及其对自由基的清除作用
Optimization of extraction technology and scavenging activity to free radicals of polysaccharides from Natural Boletus
摘要
Abstract
On the basis of single factor test,optimization of extraction process of crude Natural Boletus polysaccharides was investigated by orthogonal tests.Then the Natural Boletus polysaccharide was obtained after ethanol-precipitation,deproteinixation and vacuum drying.The results showed that the optimum conditions for the isolation was as follows:temperature was 75℃,time was 2h,material to water ratio was 1∶25,times was 3,polysaccharide yield was 9.13%.The major factors affecting Natural Boletus polysaccharides extraction were material to water ratio and temperature in the first place,and times in the next place.The scavenging ability of ·OH and O-2· of Natural Boletus polysaccharide were assayed by chemiluminescence.There results showed that Natural Boletus polysaccharide had evident abilities of scavenging hydroxyl radicals and superoxide anion radicals.The crude Natural Boletus polysaccharides obtained had strong superoxide anion radicals scavenging effect,with an IC50 of 5.40mg/mL,which was more than 1.18-fold increased after hydroxyl radicals,revealing an IC50 of 6.36mg/mL.关键词
野生牛肝菌/多糖/超氧自由基/羟自由基Key words
Natural Boletus/polysaccharides/superoxide radicals/hydroxyl radicals分类
轻工纺织引用本文复制引用
杜敏华,张英君,刘明星,冯伟伟..野生牛肝菌多糖提取工艺的优化及其对自由基的清除作用[J].食品工业科技,2012,33(22):292-295,302,5.基金项目
河南省教育厅自然科学研究计划项目 （）
南阳师范学院SPCP项目 （）
