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田口设计优化耶氏酵母生产γ-癸内酯

赵玉萍 徐岩 朱春

食品工业科技2012,Vol.33Issue(22):326-330,5.
食品工业科技2012,Vol.33Issue(22):326-330,5.

田口设计优化耶氏酵母生产γ-癸内酯

Optimization of culture medium for γ-decalactone production by Yarrowia sp.using Taguchi design

赵玉萍 1徐岩 2朱春3

作者信息

  • 1. 江南大学生物工程学院酿造科学与酶技术中心,教育部工业生物技术重点实验室,江苏无锡214122 淮阴工学院生命科学与化学工程学院,江苏淮安223003
  • 2. 江南大学生物工程学院酿造科学与酶技术中心,教育部工业生物技术重点实验室,江苏无锡214122
  • 3. 淮阴工学院生命科学与化学工程学院,江苏淮安223003
  • 折叠

摘要

Abstract

Culture medium for γ-decalacton production by Yarrowia sp.was optimized by using Taguchi design.First,single factor was tested,then orthogonal experiment was designed to carry out using Minitab software,and the result of the mean and SNR of level of each factor were analyzed by using Taguchi design.The result proved that the effect of composite nitrogen was very significant,and(NH4)2HPO4 was significant.Software predicted the optimal culture medium consisted of 20g/L wheat bran,7.5g/L yeast extract,16.5g/L(NH4)2HPO4,6g/L KH2PO4 and 0.5g/L MgSO4·7H2O,and GDL yield was 2.49g/L,the SNR was 9.04dB.The γ-decalacton yield of verification experiment was 2.65g/L,and the SNR was 9.68dB.The result showed that the optimal solution achieved the desired effect with good stability.

关键词

γ-癸内酯/耶氏酵母/田口设计/优化

Key words

γ-decalactone/Yarrowia sp./Taguchi design/medium optimization

分类

轻工纺织

引用本文复制引用

赵玉萍,徐岩,朱春..田口设计优化耶氏酵母生产γ-癸内酯[J].食品工业科技,2012,33(22):326-330,5.

基金项目

江苏省科技支撑计划 ()

淮安市工业科技计划 ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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