食品科学2012，Vol.33Issue(20)：74-79,6.
大孔吸附树脂分离纯化五倍子鞣质
Separation and Purification of Tannin from Galla chinensis by Macroporous Resin Adsorption
摘要
Abstract
NKA-2 macoporous adsorption resin was the best resin for the separation and purification of crude tannin extract from Galla chinensis as demonstrated by a comparison of the effectiveness of 8 types of macoporous adsorption resin in static adsorption and desorption of tannin. One-factor-at-a-time and orthogonal array design were used to optimize dynamic adsorption and desorption parameters for the purification of the crude tannin extract. The optimal process parameters for maximum tannin yield were determined as follows: 6 BV of 5 mg/mL sample was loaded onto the column at a flow rate of 1 BV/h and after complete adsorption, the column was then eluted with 4 BV of 80% ethanol solution at a flow rate 1 BV/h, yielding a tannin yield of 85.37% and a tannin purity of 62.99%. However, the optimal process parameters for maximum tannin purity were determined as fol ows: 5 BV of 6 mg/mL sample was loaded onto the column at a flow rate of 2 BV/h and after complete adsorption, the column was then eluted with 4 BV of 80% ethanol solution at a flow rate 3 BV/h, yielding a tannin yield of 76.97% and a tannin purity of 67.78%.关键词
五倍子/鞣质/大孔吸附树脂/分离纯化Key words
Galla chinensis/tannin/macroporous resin/separation and purification分类
化学化工引用本文复制引用
高燕,李勤,盛达成,肖文军,刘仲华,张盛,傅冬和..大孔吸附树脂分离纯化五倍子鞣质[J].食品科学,2012,33(20):74-79,6.基金项目
“十二五”国家科技支撑计划项目(2011BAD10B00)；国家农业科技成果转化资金项目(2011GB2D200010) （2011BAD10B00）
