食品科学2012，Vol.33Issue(20)：94-98,5.
基于酶法辅提和微波酸解的黄姜薯蓣皂苷元清洁生产工艺
Clean Production of Diosgenin from Dioscorea zingiberensis C. H. Wright Based on Enzymatic Treatment and Microwave-Assisted Acidolysis
摘要
Abstract
Based on the concept of clean production and comprehensive resource utilization, a new procedure for the extraction of diosgenin from Dioscorea zingiberensis C. H. Wright rhizomes was proposed. This procedure involved four stages, recovery of starch and cellulose from the raw material, one-step multi-enzymatic hydrolysis, microwave-assisted acidolysis and diosgenin extraction. First, most of the starch and cellulose in Dioscorea zingiberensis C. H. Wright rhizomes were extracted physically. In this process, ultrasonication was introduced to save water. Second, the residual starch and cel ulose were further hydrolyzed with added thermostable α-amylase and glucoamylase. Third, diosgenin was released by acidolysis. In this process, microwave heating was used to save acid and shorten the acidolysis time. Final y, a smal quantity of solvent was used to extract diosgenin and the extract was refined by re-crystallization. Compared with the traditional single acidolysis procedure, this clean production procedure could save acid by 96.97% and result in an increase in diosgenin yield of 34.56%.关键词
复合酶/微波/黄姜/皂苷元/清洁生产Key words
multi-enzyme/microwave/Dioscorea zingiberensis C. H. Wright/diosgenin分类
农业科技引用本文复制引用
李会,张佳佳,梁静怡,许正宏,史劲松..基于酶法辅提和微波酸解的黄姜薯蓣皂苷元清洁生产工艺[J].食品科学,2012,33(20):94-98,5.基金项目
国家“863”计划项目(2011AA02A211)；中央高校基本科研业务费专项(JUSRP111A50) （2011AA02A211）
