食品科学2012，Vol.33Issue(20)：153-156,4.
毛细管硅胶整体柱分离分析食品中有机酸
Separation and Analysis of Organic Acids in Food by Capillary Silica Monolithic Column
摘要
Abstract
A capil ary electrochromatographic method for the separation and analysis of organic acids in food was proposed using a capillary silica monolithic column. Optimization of experimental parameters that influence separation efficiency was carried out. A capillary silica monolithic C18 column was prepared in our lab for the separation of organic acids using a mobile phase (pH 5.2) consisting of 150 mmol/L sodium dihydrogen phosphate-60 mmol/L borax buffer solution with a final concentration of 5:2 in the mixture and 0.5 mmol/L cetyl trimethyl ammonium bromide. Other experimental conditions were as follows: voltage -6 kV; injection time 10 s; column temperature 25 ℃; and detection wavelength 200 nm. Results showed that the linear range was 10–1000 μg/mL for oxalic acid and fumaric acid, 20–500 μg/mL for succinic acid, 15–1000 μg/mL for tartaric acid and citric acid. The average recovery rates for the organic acids were between 89.8% and 101.1%, with a relative standard deviation (RSD) less than or equal to 6.23% (n= 5). The detection limits of the proposed methods were between 3.0 and 7.5 μg/mL. In conclusion, capil ary silica monolithic column is inexpensive, stable to heat and organic solvents, and repeatedly usable. Capillary electrochromatography with capillary silica monolithic column allows simple, rapid and effective separation and analysis of organic acids in food.关键词
食品/有机酸/毛细管硅胶整体柱/毛细管电色谱法Key words
food/organic acids/capillary silica monolithic column/capillary electrochromatography分类
轻工纺织引用本文复制引用
高文惠,杨桂君,贾英民..毛细管硅胶整体柱分离分析食品中有机酸[J].食品科学,2012,33(20):153-156,4.基金项目
河北省自然科学基金项目(B2008000669)；河北省科技支撑计划项目(10276902D) （B2008000669）
