食品科学2012，Vol.33Issue(20)：167-170,4.
固相萃取-流动注射化学发光法测定猪肉中盐酸克伦特罗
Determination of Clenbuterol in Pork by Solid Phase Extraction Combined with Flow Injection Chemiluminescence
摘要
Abstract
Clenbuterol is known to be able to inhibit the chemiluminescence generated from the oxidation of luminal by ferricyanatum kalium under alkaline conditions. Based on this principle, an analytical method was developed for the determination of clenbuterol in pork using solid phase extraction and flow injection chemiluminescence. Clenbuterol residue present in pork was extracted into ethyl acetate and cleaned up using a solid-phase extraction cartridge before analysis. The results indicated that the developed method was rapid and sensitive. The limit of detection was 0.026 μg/mL and the linear range was 0.05–1.0 μg/mL. The relative standard deviation (RSD) for 8 repeated determinations was 1.16%. The average recovery rate of clenbuterol in blank pork sample was 91.96%, which was comparable to the results obtained by GC-MS or HPLC.关键词
固相萃取/流动注射/化学发光/盐酸克伦特罗Key words
solid phase extraction/chemiluminescence/flow injection/clenbuterol分类
化学化工引用本文复制引用
哈婧,王秋平,张佳丽..固相萃取-流动注射化学发光法测定猪肉中盐酸克伦特罗[J].食品科学,2012,33(20):167-170,4.基金项目
河北省自然科学基金项目(B2010000849)；国家自然科学基金青年科学基金项目(21102032) （B2010000849）
