食品与发酵工业2012，Vol.38Issue(10)：29-34,6.
以蔗糖为底物双酶法合成直链糊精
Enzymatic Synthesis of Amylodextrin from Sucrose
摘要
Abstract
Sucrose phosphorylase and glucan phosphorylase were chosen to synthesize the amylodextrin and the process conditions were optimized in this paper. Using sucrose as substrate and phosphate as buffer, the reaction was catalyzed by sucrose phosphorylase to produce the intermediate product glucose-l-phosphate which was determined by liquid chromatography-mass spectrometry. Based on the above system, the synthetic reaction using mahotetraose as primer was catalyzed by glucan phosphorylase to produce amylodextrin. The degree of polymerization of the product contained in the system was determined by high-performance anion-exchange chromatography. The optimal conditions including enzyme concentration, time, pH and temperature were determined by mono-factor experiment. The results indicated that the optimum conditions of the reaction which using sucrose phosphorylase as catalyst to produce glucose- 1-phosphate were as follows: enzyme concentration 4.0 U/mL, reaction time 4 h, pH 6.5, temperature 40℃ ; and conditions of reaction which using glucan phosphorylase as catalyst to synthesis amylodextrin were as follows: enzyme concentration 5.0 U/mL, reaction time 4 h, pH 4.5, temperature 40℃.关键词
酶法合成/蔗糖磷酸化酶/葡聚糖磷酸化酶/直链糊精Key words
enzymatic synthesis/sucrose phosphorylase/glucan phosphorylase/amylodextrin分类
轻工纺织引用本文复制引用
李恬,周星,徐进,徐学明,金征宇..以蔗糖为底物双酶法合成直链糊精[J].食品与发酵工业,2012,38(10):29-34,6.基金项目
国家自然科学基金 （）
