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用化学发光法分析燕麦发芽前后酚类物质抗氧化活性

付晓燕 吴茜 隋勇 谢笔钧 孙智达

食品与发酵工业2012,Vol.38Issue(10):63-69,7.
食品与发酵工业2012,Vol.38Issue(10):63-69,7.

用化学发光法分析燕麦发芽前后酚类物质抗氧化活性

The Comparison of Antioxidant Activity of Phenols in Oat After Germination by Chemiluminescence Method

付晓燕 1吴茜 2隋勇 2谢笔钧 2孙智达2

作者信息

  • 1. 华中农业大学楚天学院食品与生物科技学院,湖北武汉430205
  • 2. 华中农业大学食品科技学院,湖北武汉430070
  • 折叠

摘要

Abstract

Oat seeds were soaked the bud of 6d and the root of 6d were and germinated. Phenol compounds from non-germination oat, the grain of 6d, extracted and purified. Antioxidant activities of purified products from different parts were studied. The results indicated that the purified products from non-germination oat, the grain of 6d, the bud of 6d and the root of 6d showed strong radical scavenging activity in various antioxidant assays. The IC50 of O2- · scavenging effect were 0. 952 mg/mL, 0. 702mg/mL, 3. 248mg/mL, 1. 060mg/mL respectively. The IC50 of · OH scavenging effect were 8. 80μg/mL, 3.3μg/mL 13. 69μg/mL, 11.68 μg/mL respectively. The IC50 of H2O2 scav- enging effect were 10.97μ, 8.64μg/mL, 13.05μg/mL, 9.6μg/mL respectively. The antioxidant activity of oat grain was increased to a certain extent during oat germination, while the antioxidant activity of oat bud was lower than oat grain and root.

关键词

燕麦/发芽/酚类物质/化学发光法/抗氧化活性

Key words

oat/germination/phenols/chemiluminescence method/antioxidant activity

分类

轻工纺织

引用本文复制引用

付晓燕,吴茜,隋勇,谢笔钧,孙智达..用化学发光法分析燕麦发芽前后酚类物质抗氧化活性[J].食品与发酵工业,2012,38(10):63-69,7.

基金项目

“十一五”国家科技支撑计划项目 ()

食品与发酵工业

OA北大核心CSCDCSTPCD

0253-990X

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