茶叶科学2012，Vol.32Issue(6)：500-508,9.
茶树泛素活化酶基因全长cDNA克隆及序列分析
Cloning and Sequencing of UBA1 Gene Full-length cDNA from Tea Plant
摘要
Abstract
The cDNA-AFLP technology was applied to analyze gene expression during periodic albinism process of Anji Baicha. Some transcript-derived fragments (TDFs) were isolated occurring in both the albinistic and re-greening stage leaves. One of them showed a high similarity to ubiquitin-activating enzyme 1 (UBA\) gene. Based on the fragment, the full length of UBAl gene with 3 764 bp (GenBank Accession No. JN180299) cDNA was obtained via rapid amplification of cDNA ends (RACE), named Camellia Sinensis UBA1 gene. It contained an open reading frame (ORF) encoding a polypeptide of 1 094 amino acid residues with a predicable molecular mass of 121 kD. Analysis of the nucleotide sequence and deduced amino acid sequence showed 82%, 81%, 79%, 79%, 77% homology with UBAl genes from Nicotiana tabacum, Ricinus communis, Oryza saliva subsp. Japonica, Triticum aestivum, Arabidopsis thaliana, respectively. Analysis by qRT-PCR showed that the transcript of UBAl was significantly up-regulated at the albinistic stage to 2.49-fold higher than that at the re-greening stage. This is a key enzyme in the ubiquitin-proteasome mediated protein degradation system. The clone and analysis of the tea plant UBAl gene establishes a good foundation for further study on the molecular mechanism of periodic albinism in Anji Baicha.关键词
茶树/泛素活化酶基因/cDNA克隆/序列分析Key words
Camellia sinensis (L.) O. Kuntze, Ubiquitin-Activating Enzyme l(UBAl), cDNA cloning, sequence analysis分类
农业科技引用本文复制引用
邓婷婷,吴扬,李娟,李银花,黄建安,刘仲华..茶树泛素活化酶基因全长cDNA克隆及序列分析[J].茶叶科学,2012,32(6):500-508,9.基金项目
国家科技支撑计划(2011BAD01B01)、国家自然科学基金(30871572)、湖南农业大学人才稳定基金(07WD22) （2011BAD01B01）
