茶叶科学2012，Vol.32Issue(6)：509-516,8.
生长素相关基因在茶树腋芽冬季休眠不同生长阶段的表达分析
Expression Analysis of Auxin-related Genes at Different Winter Dormant Stages of Axillary Buds in Tea Plant(Camellia sinensis)
摘要
Abstract
The expression patterns of some auxin-related genes, including auxin response factors (ARFs), primary auxin responsive genes, auxin-regulated genes, auxin influx carrier gene and auxin-binding protein (ABP) gene, were studied in different stages of tea plant axillary bud dormancy and its release. The results showed that (1) the expression levels of five in six ARF homologous EST fragments were up-regulated in the deep dormancy and down-regulated after sprouted, the level of the last one showed the opposite expression levels. (2) There were significant differences among the expression patterns of 3 ESTs which are homologous with primary auxin responsive genes. EST which is homologous with SAURs was up-regulated in the dormancy stage but down-regulated after dormancy release. On the contrary, the 2 ESTs which are homologous with GH3 and Aux/IAAs were up-regulated in sprouting stage while down-regulated in dormant stages and after sprouting stages. (3) The CsGLPl gene which was homogenous with ABPs has a lower expression level in the dormancy stage but maintain rising trend after germination.(4) The EST which is homogenous with auxin influx carrier gene showed the highest expression levels during the deep dormant stage but maintain lower levels in other stages. (5) Two auxin-regulated ESTs showed different expression patterns. The expression levels of auxin-induced EST were higher in the dormant stages than those in the sprouting stages, while the levels of the other auxin-repressed EST were higher in the sprouting stage than that in the dormant stages. These results indicated that auxin promote the dormancy of tea axillary buds in winter.关键词
茶树(Camellia sinensis)/芽休眠与萌发/生长素相关基因/表达分析Key words
tea plant (Camellia sinensis), bud dormancy and budbreak, auxin-related gene, expression analysis分类
农业科技引用本文复制引用
王新超,马春雷,杨亚军,曹红利,郝心愿,金基强..生长素相关基因在茶树腋芽冬季休眠不同生长阶段的表达分析[J].茶叶科学,2012,32(6):509-516,8.基金项目
现代农业(茶叶)产业技术体系项目(CARS-23)、国家自然科学基金项目(30872059)资助 （茶叶）
