现代食品科技2012，Vol.28Issue(11)：1454-1458,5.
阪崎肠杆菌随机突变体库的构建
Generation of a Cronobacter (Enterobacter sakazakii)Mutant Library
摘要
Abstract
Cronobacter (Enterobacter sakazakii), a pathogen commonly found in powdered infant formula (PIF), is an important food-borne pathogen. At present, very limited information is available regarding the function of genes, virulence factors and the pathogenesis of Cronobacter. In this study, plasmid pTnMod-okm and pBSL180 were used to build Cronobacter random mutant library. Finally, the mutant library of Cronobacter was built based on transposon that contained in pBSL180 plasmid of Escherichia coli WM3064 through Chromogenic technology and antibiotic resistance labeling (kanamycin 50 μg/tnL). In subsequence research, gene assignment and finding the similar function gene can be achieve, which layed the foundation for further studying the resistance mechanism and the pathogenesis of Cronobacter.关键词
阪崎肠杆菌/转座子/突变体库Key words
Cronobacter (Enterobacter sakazakii)/ transposon/ mutant library引用本文复制引用
董晓晖,吴清平,莫树平,杨小鹃..阪崎肠杆菌随机突变体库的构建[J].现代食品科技,2012,28(11):1454-1458,5.基金项目
广东省科技计划项目(2008A030203012) （2008A030203012）
广东省中国科学院全面战略合作专项(2010B090301037) （2010B090301037）
