现代食品科技2012，Vol.28Issue(11)：1572-1574,1580,4.
早花及晚花灰毡毛忍冬中绿原酸含量分析
Analysis on the Content of Chlorogenic Acid from Early-flowering and Later-flowering Lonicera Macranthoides
摘要
Abstract
To explore suitable planting fine Lonicera macranthoides varieties in north Guizhou, the content of chlorogenic acid in flower buds and leaves of early-flowering and later-flowering varieties were researched. Chlorogenic acid were obtained with ultrasonnic wave-assisted extraction technique, and analyzed using HPLC methods with operating condition as follows: Diamonsil (5 urn, C18(2), 250 mm×4.6 mm),the volume flow l.0mL/min, the column temperature 40°C and the detection wavelength 325nm. The mobile phase methanol/acetonitrile (5:ll)-0.1% phosphoric acid=20:80. The result showed that the content of chlorogenic acid ranged from 2.36% to 3.44% in flower buds and from 0.49% to 1.11% in leaves of early-flowering Lonicera macranthoides. In flower buds and leaves of later-flowering one, it as in the range of 2.60%~4.07% and 1.92%~2.83%, respectively. It was concluded that fee later-flowering Lonicera macranthoide was a rich-chlorogenic acid breeding strain.关键词
灰毡毛忍冬/早花/晚花/绿原酸/高效液相色谱Key words
Lonicera macranthoides Hand.-Mazz/ early-flowering/ later-flowering/ chlorogenic acid/ HPLC引用本文复制引用
余正文,刘方方,何磊磊,玉秋萍,张妮,宋庆发,乙引..早花及晚花灰毡毛忍冬中绿原酸含量分析[J].现代食品科技,2012,28(11):1572-1574,1580,4.基金项目
国家自然科学基金(31060056) （31060056）
贵州师范大学博士科研项目(11904-05032110014) （11904-05032110014）
贵州省中药现代化专项(黔科合中药字[2011]5046号) （黔科合中药字[2011]5046号）
贵州省科技基金项目(黔科合J字[2011]2368号) （黔科合J字[2011]2368号）
贵州省科技创新人才团队建设项目(黔科合人才团队[2009]4007号) （黔科合人才团队[2009]4007号）
