现代食品科技2012，Vol.28Issue(11)：1595-1599,5.
胶体金免疫层析技术快速检测沙丁胺醇残留
Colloidal Gold Immunochromatographic Assay for Residual Sabutamol
吴巧丽 1叶春生2
作者信息
- 1. 浙江省质量检测科学研究院,浙江杭州310013
- 2. 杭州博拓生物技术有限公司,浙江杭州311121
- 折叠
摘要
Abstract
Colloidal gold immunochromatography assay can rapidly test residual subutamol in meat and animal feed. An anti-salbutamol antibody is conjugated to colloidal gold and placed on conjugate pad. Salbutamol-BSA antigen is coated on nitrocellulose membrane as test line (T line). Colloidal gold provides color to visualize antibody-antigen binding. If there is Salbutamol in the sample, it will bind gold-antibody conjugate and prevent its binding onto the Salbutamol-BSA antigen in the test line(T line) on membrane. As result, no color will be visible on test line on membrane. Otherwise, anti-salbutamol antibody conjugated to colloidal gold will bind the Salbutamol-BSA antigen leading to red color on test line. The color of test line indicates positive or negative result. With optimized reagents, the lowest limit of detection is 2ng/ml. The cross reaction with Clenbuterol is 2.67%. Results show in 3 minutes and are visible to naked eyes. This method provides a sensitive and specific detection approach for the presence of salbutamol in meat and animal feed on site.关键词
沙丁胺醇/免疫层析/快速检测/胶体金/竞争法Key words
salbutamol/ immunochromatographic assay/ rapid test/ colloidal gold/ competition method引用本文复制引用
吴巧丽,叶春生..胶体金免疫层析技术快速检测沙丁胺醇残留[J].现代食品科技,2012,28(11):1595-1599,5.
