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测定雌马酚的紫外法建立与应用

李笑梅 张娜 贾冰心

食品工业科技2012,Vol.33Issue(24):52-54,3.
食品工业科技2012,Vol.33Issue(24):52-54,3.

测定雌马酚的紫外法建立与应用

Establishment and application of equol determination by ultraviolet spectrophotometry method

李笑梅 1张娜 1贾冰心1

作者信息

  • 1. 哈尔滨商业大学省高校食品科学与工程重点实验室,黑龙江哈尔滨150076
  • 折叠

摘要

Abstract

A UV spectrophotometer used for determination of equol was established. Using equol standard and the urine equol from the tester who ate the soybean isoflavones for samples,the ultraviolet method character of determine equol was studied through UV spectrophotometer to determine the detection limit and quantitative limit. The result was as follows.the detection limit was 5×10^-10g/mL,the quantitative limit was 15×10^-10g/mL. Application for equol measurement of human urine,the precision was 4.45% and the average recovery rate was 100.25%,the result was reliable. The new UV method conforms to the quantitative analysis of the requirements was,it had advantages of simple,rapid,and high sensitivity. It was suitable for soybean isoflavone and equol established measuirement application.

关键词

雌马酚/紫外法/尿样

Key words

UV detection/equol/urine samp

分类

轻工纺织

引用本文复制引用

李笑梅,张娜,贾冰心..测定雌马酚的紫外法建立与应用[J].食品工业科技,2012,33(24):52-54,3.

基金项目

基金项目:黑龙江省高校科技创新团队建设计划资助项目(2010td04). ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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