食品工业科技2012，Vol.33Issue(24)：143-146,4.
麻栎叶黄酮的大孔树脂分离及其对α-葡萄糖苷酶活性的抑制作斥
Flavonoids extracted from Quercus acutissima Carr. leaves purification and inhibit the activity of α-glucosidase
摘要
Abstract
The macroporous resins was selected to study the purification of the flavonoids from Quercus acutissima Carr. leaves and inhibitory activities against α-glucosidase. Screening was performed to choose the best resin by comparing the resins static dynamic adsorption experiments. The results indicated that X-5 macroporous resins had better adsorption and desorption property for purification. The bsst condition of X-5 macroporous resins was initial solution concentration about 52.79μg .mL-l,initial solution amount 50mL,the sample speed at 0.5mL.min-l,pH5.0. the elution solvent 30mL of 95% ethanol the flow rate at 1.0mL.min-L Under above condition,the purity of flavonoids could reach to 58.33%. Flavonoids of Quercus acutissirna Carr. leaves showed potent inhibitory activities against a-glucosidase with IC50 values of about 13.11μg/mL.关键词
麻栎叶/黄酮/大孔树脂/纯化/α-葡萄糖苷酶Key words
Quercus acutissima Carr. leaves/flavonoids/macroporous resins/purification α-glucosidase分类
农业科技引用本文复制引用
罗侠,孙艳辉,刘文娟,刘克忠..麻栎叶黄酮的大孔树脂分离及其对α-葡萄糖苷酶活性的抑制作斥[J].食品工业科技,2012,33(24):143-146,4.基金项目
基金项目：安徽省高校省级自然科学研究项目（KJ20112282）. （）
