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降胆固醇优良菌株的筛选及其胆盐水解酶特性研究

李婷婷 张洋 林超 霍贵成

食品工业科技2012,Vol.33Issue(24):191-194,4.
食品工业科技2012,Vol.33Issue(24):191-194,4.

降胆固醇优良菌株的筛选及其胆盐水解酶特性研究

Screening of excellent strains for cholesterol-lowing and studies on characteristics of bile salt hydrolase

李婷婷 1张洋 1林超 1霍贵成1

作者信息

  • 1. 乳品科学教育部重点实验室,东北农业大学,黑龙江哈尔滨150030
  • 折叠

摘要

Abstract

Three strains of lactic acid bacteria with cholesterol removal ability and bile salt hydrolase (BSH) producing activity were further filtered, using the standard which was included three indexes of the tolerance to simulated artificial gastric juice,hydrophobic capability and deconjugation of sodium taurocholate. A potential strain of lactic acid bacteria with comprehensive performance and excellent cholesterol-lowering ability was filtered out,and partial BSH characteristics was studied. The result indicated that Enterococcus faecium 90-1 could be potential cholesterol-lowering strain for further research with good comprehensive performance. The optimum reaction pH of BSH was 6.0,the optimum reaction temperature was 37℃ ,the optimum substrate concentration was 7mmol/L,and the optimum ATP concentration was 2.5mg/mL.

关键词

降胆固醇/屎肠球菌/胆盐水解酶/特性

Key words

cholesterol removal/Enterococcus faecium/bile salt hydrolase/characteristics

分类

轻工纺织

引用本文复制引用

李婷婷,张洋,林超,霍贵成..降胆固醇优良菌株的筛选及其胆盐水解酶特性研究[J].食品工业科技,2012,33(24):191-194,4.

基金项目

基金项目:教育部长江学者和创新团队发展计划资助(IRT0959,2011AA100902). ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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