食品工业科技2012，Vol.33Issue(24)：210-214,5.
具有高效抑菌活性的肠道乳酸菌的筛选
Selection of high-efficiant antibacterial intestinal lactic acid becteria
摘要
Abstract
Objective: 19 strains of intestinal bacteria from the elderly and 8 strains from common health people as the experiment object,screening had hingh efficient antibacterial strain and determining the antibacterial effect of suspicious substance. Methods:Using filter paper method for screening high antibacterial activity strains, at the same time by different treatment (temperature, acidity, concentration of the enzyme) determination the changes of antibacterial activity,finally determination of the content of organic acid by using high performance liquid chromatography method and determination the inhibiting ability of organic acids. Results: From 27 strains breeding of a three strain with broad-spectrum,highly efficient inhibition effect of lactic acid bacteria,studing the bacteriostatic compositions were three strains of lactic acid bacteria,finally found the antibacterial effect of material were bacteriocins and short chain organic acid. Conclusion:27 bacterial strains had the ability of antibacterial ,but the ability of antibacterial of bacterial strains separated from dung of long- lived old people was stronger than the general people's. We conducted antibacterial test by using these metabolites of lactobacillus-organic acid (Lactic acid,butyric acid and acetic acid) and bacteriocins(stable to temperature and sensitive to protease) as main substrate. The result showed the two kinds of substances had significantly antibacterial effects at the low pH condition,which was essential condition to antibacterial activity.关键词
乳酸菌/抑菌作用/细菌素/有机酸Key words
lactic acid becteria/bacteriostasis/bacteriocin/organic acid分类
轻工纺织引用本文复制引用
麦热姆妮萨·艾麦尔,乌斯满·依米提,阿迪拉·多力坤,多力坤·麦麦提优素莆..具有高效抑菌活性的肠道乳酸菌的筛选[J].食品工业科技,2012,33(24):210-214,5.基金项目
基金项目：新疆特殊环境微生物实验室开放课题基金 （）
中国科学院心理研究所合作项目（140696）. （）
