食品工业科技2012，Vol.33Issue(24)：246-248,388,4.
高产洛伐他汀红曲菌株的诱变选育
Breeding of high-lovastatin Monascus strains
摘要
Abstract
Using Monascus Purpureus HW as original strain, LiCI mutation breading was conducted for increasing the production of Iovastatin. The optimal amount of LiCl(lmol/L) was the 200μL. Six of high-lovastatin mutants were respectively obtained through determination of Iovastatin in the broth by HPLC. Among these mutants,the yield of Iovastatin by HW3,HW5 and HW6 reached 35.94,38.86 and 30.07mg/L,which were 214% ,239% and 169% higher than that by the original strain,respectively. And the development of colony morphologies indicated that there was a positive correlation between the capacity of Iovastatin and pigment among these mutants. Furthermore,colonies with or without folds also affect the performance of Iovastatin production. The yield of Iovastatin by HW3,HW5 and HW6 decreased by 11.8% ,14.9% and 32.1% ,after subculture for 10 times, respectively. Therefore,the HW3 and HW5 mutants are stable strains that are worth further study.关键词
红曲霉菌/氯化锂/洛伐他汀/菌株选育Key words
Monascus Purpureus/LiCI/lovastatin/strain screening分类
轻工纺织引用本文复制引用
李滔滔,李小龙,张凤琴..高产洛伐他汀红曲菌株的诱变选育[J].食品工业科技,2012,33(24):246-248,388,4.基金项目
基金项目：湖南省科技厅支撑计划重点项目 （）
湖南省博士后计划项目 （）
中国博士后基金（2011M501309,2012T50713）. （）
