食品科学2012，Vol.33Issue(21)：11-15,5.
灵菌红素与肌红蛋白相互作用的光谱分析
Spectroscopic Analysis of the Interaction of Prodigiosin with Myohemoglobin
摘要
Abstract
The interaction between myohemoglobin(Mb) and prodigiosin(PG) was investigated by UV-visible,fluorescence,synchronous fluorescence,FT-IR and circular dichroism(CD) spectroscopic techniques.The UV absorption of Mb was increased in the presence of PG,indicating that PG interacted mainly with amino acid residues on the outside of Mb.The peak fluorescence intensity of Mb was increased through its interaction with PG.Moreover,the binding constant between them was decreased with increasing temperature.The enthalpy change(ΔH) was-25.293 kJ/mol,and the entropy change(ΔS) was-10.238 J/(mol?s) at 298 K and-10.239 J/(mol?s) at 310 K as calculated according to the thermodynamic equation.The data showed that Van der Waals force played a critical role in the interaction between Mb and PG.Synchronous fluorescence spectroscopic analysis revealed alteration of the microenvironment of amino acid residues as a result of the interaction between both materials.FT-IR analysis showed alternation of the secondary structure of Mb induced by the interaction of amide groups in its polypeptide chains with PG.CD analysis demonstrated a reduction in α-helix content of Mb due to PG-induced alteration of the secondary structure.关键词
灵菌红素/肌红蛋白/紫外-可见光谱/荧光光谱/红外光谱/圆二色谱Key words
prodigiosin/myohemoglobin/UV-visible absorption/fluorescence spectrum/FT-IR/circular dichroism分类
轻工纺织引用本文复制引用
高园园,金星,丁军,刘淑超,章西海,康乐,唐静,杨启银..灵菌红素与肌红蛋白相互作用的光谱分析[J].食品科学,2012,33(21):11-15,5.基金项目
国家自然科学基金项目（30970523 （）
30770073） （）
江苏高校优势学科建设工程资助项目 （）
