食品科学2012，Vol.33Issue(21)：173-176,4.
18S rDNA和ITS相结合鉴定一株盐生海芦笋内生真菌的研究
Identification of a Halophilic Endophytic Fungus Isolated from Salicornia bigelovii
摘要
Abstract
In the current study,high-xylanase-producing strain was screened from different soil samples by transparent circle method,using xylan as the only carbon source in medium.The cultural condition for xylanase production by strain L10608 was optimized and the hydrolysis property of the enzyme was further investigated.The results indicated that the optimum fermentation medium contained a carbon source of water-insoluble xylan(80 mesh) of 25 g/L,compound nitrogen source of soya peptone of 25 g/L and yeast extract of 5 g/L,initial pH 6.0,cultural temperature 40 ℃,rotational speed of 200 r/min,surfactant polysorbate 80 of 4 g/L.Under the optimized condition,the enzyme activity reached 1074.8 U/mL.The xylanase utilized all birch wood xylan,beech wood xylan and oat xylan as the substrate,exhibiting that xylanase produced by L10608 was endo-xylanase with xylobiose and xylotriose as the major hydrates.These results showed that strain L10608 could hopefully be used for industrial production of functional xyloolygosaccharides.关键词
海芦笋/鉴定/18S/rDNA基因/ITS基因/系统发育分析Key words
actinomycetes/xylanase/liquid fermentation/optimize/xyloolygosaccharides分类
轻工纺织引用本文复制引用
王惠,湛东锐,李连强,曹娜,赵育卉,刘天行,孟晓露,辛志宏..18S rDNA和ITS相结合鉴定一株盐生海芦笋内生真菌的研究[J].食品科学,2012,33(21):173-176,4.基金项目
国家自然科学基金面上项目 （）
2011年国家大学生创新训练计划项目 （）
