食品科学2012,Vol.33Issue(21):226-230,5.
金针菇液体发酵产漆酶培养条件的研究
Optimization of Liquid Culture Conditions for Laccase Production by Flammulina velutipes LP03
摘要
Abstract
The effect of carbon resource,nitrogen source,Cu2+ and other factors on laccase secretion by and cell growth of Flammulina velutipes LP03 were investigated.The optimum conditions were 3 g/100 mL corn flour,1 g/100 mL peptone,0.5 g/100 mL glucose,1 mmol/L copper sulfate,initial pH 6.0,200 mL/500 mL liquid medium,28 ℃ and 160 r/min.The maximal enzyme activity was(1813.52 ±5.59) U/L,which was 3.78 times of that before optimization.关键词
金针菇/漆酶/培养条件Key words
Flammulina velutipes/laccase/culture condition分类
轻工纺织引用本文复制引用
刘苹,李刚,温少红..金针菇液体发酵产漆酶培养条件的研究[J].食品科学,2012,33(21):226-230,5.基金项目
山东省优秀中青年科学家科研奖励基金项目 ()
山东省科技攻关计划项目 ()