食品科学2012，Vol.33Issue(21)：249-252,4.
磁性壳聚糖微球固定化碱性蛋白酶的酶学性质
Enzymatic Properties of Magnetic Chitosan Microspere-Immobilized Alkaline Protease
摘要
Abstract
Alkaline protease was immobilized onto magnetic chitosan microspheres prepared from Fe3O4 and chitosan by crosslinking with glutaraldehyde and its appearance and structural properties were analyzed.The results showed that the magnetic chitosan microspheres had spherical appearance with a particle size increasing from 15 to 20 nm after immobilization.FT-IR analysis revealed that Fe3O4 was well wrapped up in the magnetic chitosan microspheres.The magnetic chitosan microspheres had complete crystal structure,good magnetic response and strong superparamagnetic behaviors before and after immobilization of alkaline protease.The optimal reaction temperature and pH for the immobilized enzyme were 50 ℃ and 11 compared with 60 ℃ and 10,respectively,for the free enzyme.The Km was 5.85 ×10-4 mg/mL for the immobilized enzyme and 6.06 ×10-4 mg/mL for its free counterpart.关键词
壳聚糖/磁性/固定/碱性蛋白酶/酶学性质Key words
magnetism/chitosan/immobilization/alkaline protease/enzymatic property分类
生物科学引用本文复制引用
李丹丹,江连洲,李杨,王胜男,王梅,李丹..磁性壳聚糖微球固定化碱性蛋白酶的酶学性质[J].食品科学,2012,33(21):249-252,4.基金项目
黑龙江省科技攻关计划项目 （）
国家现代农业产业技术体系建设专项 （）
