食品科学2012，Vol.33Issue(22)：27-32,6.
明日叶叶水溶性总黄酮提纯工艺
Extraction and Purification of Water-Soluble Flavonoids from Angelica keiskei Koidzumi Leaves
摘要
Abstract
In the present study, three aqueous extraction techniques, water bath extraction, direct heating extraction and microwave-assisted extraction were compared to determine the most suitable one for maximum extraction yield of water-soluble flavonoids from Angelica keiskei Koidzumi leaves. The water bath extraction method provided maximum extraction of water-soluble flavonoids and the optimal extraction conditions were heating at 95℃for 10 min. Further investigations were carried to explore the purification of the resulting crude extract under various conditions of macroporous resin type, sample loading amount, and elution solvent (ethanol) pH, ethanol concentration and elution volume. HP-20 type resin was found to have higher adsorption and desorption capacities for flavonoids and the optimal conditions for purifying water-soluble flavonoids from Angelica keiskei Koidzumi leaves using the resin were determined as follows: 40 mL of crude extract sample at 2.445 mg/mL were loaded onto HP-20 type resin and then desorbed with 8 BV of 70%acidified ethanol (pH 2), resulting in a purification factor of approximately 6.8.关键词
明日叶/水溶性总黄酮/大孔树脂/水浸提/提纯Key words
Angelica keiskei Koidzumi/water-soluble lfavonoids/macroporous resin/water extraction/puriifcation分类
医药卫生引用本文复制引用
郭晓青,吴金鸿,周焱富,王正武,杨科峰..明日叶叶水溶性总黄酮提纯工艺[J].食品科学,2012,33(22):27-32,6.基金项目
国家自然科学基金青年科学基金项目(31000814；31000800)；国家自然科学基金面上项目(31171642)；科技部农业成果转化项目(2011GB2C000008)；上海市科学技术委员会项目(10DZ0504000；1052NM06700)；上海交通大学医工交叉项目(YG2011MS64) （31000814；31000800）
