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响应面法优化菜籽饼粕多糖水酶法提取工艺

陈艳伟 王承明

食品科学2012,Vol.33Issue(22):44-47,4.
食品科学2012,Vol.33Issue(22):44-47,4.

响应面法优化菜籽饼粕多糖水酶法提取工艺

Optimization of Aqueous Enzymatic Extraction of Polysaccharides from Rapeseed Meal by Response Surface Methodology

陈艳伟 1王承明1

作者信息

  • 1. 华中农业大学食品科学技术学院,农业部食品安全评价重点开放实验室,湖北 武汉 430070
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摘要

Abstract

The aqueous enzymatic extraction of polysaccharides from rapeseed meal was optimized by response surface methodology. One-factor-at-a-time method was employed to investigate the effects of five operating parameters on polysaccharide yield. The optimum ratio of water to material was 25:1 (mL/g). Further, a quadratic polynomial regression model describing polysaccharide yield as a function of enzyme dosage, pH, temperature and extraction time was developed using the SAS software based on a Box-Behnken experimental design. The results of response surface analysis showed that the optimal extraction conditions were extraction at 62℃and pH 6.7 for 79 min with an enzyme dosage of 256 U/g, resulting in a polysaccharide yield as high as 9.01%.

关键词

响应面分析/菜籽饼粕多糖/提取/优化

Key words

response surface analysis/polysaccharides from rapeseed meal/extraction/optimization

分类

化学化工

引用本文复制引用

陈艳伟,王承明..响应面法优化菜籽饼粕多糖水酶法提取工艺[J].食品科学,2012,33(22):44-47,4.

基金项目

中央高校基本科研业务费专项(2011JC025) (2011JC025)

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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