食品科学2012，Vol.33Issue(22)：67-71,5.
非水相生物催化制备甘油磷酸胆碱
Biocatalytic Production of Glycerophosphatide Choline in Non-aqueous Phase
摘要
Abstract
s:This study was undertaken to optimize reaction conditions for the biocatalytic deacylation of phosphatidylcholine (PC) by lipase to glycerol-3-phosphocholine (GPC) in non-aqueous phase. The optimal reaction conditions were found to be reaction at 40℃and pH 5.0 for 6 h with an enzyme dose of 12.5 mg/mL in the presence of 4% water. Moreover, in order to increase the utilization rate of lipase and reduce solvent consumption, additional PC at a concentration of 0.437 g/mL was added in a proportion of 50%relative to the initial amount after 1.5 h during the reaction process. Under these process conditions, the average yield of GPC after aqueous extraction of reaction products was 97%(n=3) as determined by HPLC. Thus, a feasible method to prepare GPC has been established.关键词
脂肪酶/卵磷脂/甘油磷酸胆碱/非水相Key words
lipase/phosphatidylcholine/glycerophosphatide choline/non-aqueous phase分类
轻工纺织引用本文复制引用
毛慧芳,张洪斌,赵艳艳,胡雪芹..非水相生物催化制备甘油磷酸胆碱[J].食品科学,2012,33(22):67-71,5.基金项目
安徽省长三角科技联合攻关项目(10140702001) （10140702001）
