食品科学2012，Vol.33Issue(22)：154-157,4.
响应面优化草鱼鱼鳔酶溶胶原蛋白的提取工艺
Optimization of Enzymatic Extraction Process for Collagen from Grass Carp Bladders by Response Surface Methodology
任国艳 1詹永献 1郭金英 1梁旺春 1刘志龙 1史明科1
作者信息
- 1. 河南科技大学食品与生物工程学院，河南 洛阳 471003
- 折叠
摘要
Abstract
The pepsin-catalyzed hydrolysis of grass carp bladders was optimized by response surface methodology to improve the extraction yield of collagen. We explored the effects of hydrolysis parameters, enzyme loading, hydrolysis time and liquid-to-solid ratio on the extraction yield of collagen. Their optimal values for collagen extraction were found to be 40 mg/g, 48 h and 80:1 (mL/g), respectively. Under the optimized conditions, the actual extraction yield of collagen was 17.82%, which was close to the predicted value (18.04%). The results of analysis of variance showed that the fitted regression model was reliable, and each hydrolysis parameters had a significant effect on the extraction yield of collagen.关键词
响应面优化/胃蛋白酶/草鱼鱼鳔/胶原蛋白Key words
response surface methodology/pepsin/grass carp bladders/collagen分类
轻工纺织引用本文复制引用
任国艳,詹永献,郭金英,梁旺春,刘志龙,史明科..响应面优化草鱼鱼鳔酶溶胶原蛋白的提取工艺[J].食品科学,2012,33(22):154-157,4.
