食品科学2012，Vol.33Issue(22)：231-234,4.
分子荧光法分别测定4种野生可食植物中的色氨酸和酪氨酸
Determination of Tryptophan and Tyrosine in Four Edible Wild Plants by Molecular Fluorescence Spectrometry
摘要
Abstract
A new method was established to determine tryptophan and tyrosine in four edible plants wildly grown in Zhengzhou region, Sonchus asper, Plantago asiatica, Vigna minima and Pyracantha fortuneana. Samples were hydrolyzed with with 6 mol/L sodium hydroxide solution determined by molecular fluorescence spectrometry. The average contents of tryptophan and tyrosine in the wild plants were 1.37–2.63 mg/g and 7.98–25.83 mg/g, respectively, with a relative standard deviation equal to or less than 5.4%(n=5). The proposed tryptophan standard curve showed good linear relationship over the concentration range of 0.00–0.10μg/mL. The method exhibited a limit of detection of 0.011μg/mL and 0.033μg/mL and a limit of quantification of 0.037μg/mL and 0.11μg/mL for tryptophan and tyrosine, respectively. The propose tyrosine standard curve displayed a linear range of 0.0–1.0μg/mL. The average spike recovery rates of tryptophan and tyrosine in these four plants were 93.5%–105%and 94.6%–100.5%, respectively. This method was simple, sensitive, rapid, and suitable for practical applications.关键词
分子荧光法/色氨酸/酪氨酸/续断菊/车前草/贼小豆/火棘果Key words
molecular fluorescence spectrometry/tryptophan/tyrosine/Sonchus asper/Plantago asiatica/Vigna minima/Pyracantha fortuneana分类
化学化工引用本文复制引用
高向阳,张凯歌,孔欣欣,游新侠..分子荧光法分别测定4种野生可食植物中的色氨酸和酪氨酸[J].食品科学,2012,33(22):231-234,4.基金项目
河南省重点学科建设基金项目(10466-X-082301) （10466-X-082301）
