食品科学2012，Vol.33Issue(22)：242-245,4.
冬虫夏草实时荧光PCR检测方法的建立
A Real-Time PCR Method for Detection of Cordyceps sinensis (Berk.) Sacc.
摘要
Abstract
An efifcient and sensitive method was established to detect Cordyceps sinensis (Berk.) Saccardo using real-time PCR. The internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) in Cordyceps sinensis (Berk.) Saccardo. was used as a target molecular marker and sequence homology analysis was carried out on various Cordyceps species. A set of speciifc primer and probe for real-time PCR was designed which allowed the ampliifcation of 80-bp DNA fragments. The detectable DNA template concentration of the real-time PCR method for Cordyceps sinensis (Berk.) Saccardo was as low as 0.0001 ng/μL.关键词
冬虫夏草/实时荧光PCR/鉴别Key words
Cordyceps sinensis/real-time PCR/identiifcation分类
生物科学引用本文复制引用
马骏,谢力,陈永红,胡学难,高东微..冬虫夏草实时荧光PCR检测方法的建立[J].食品科学,2012,33(22):242-245,4.基金项目
广东出入境检验检疫局科技计划项目(2005GDK34) （2005GDK34）
