食品科学2012，Vol.33Issue(22)：282-285,4.
固相萃取-高效液相色谱法测定水产品中的甲基睾酮残留量
SPE-HPLC Determination of 17α-Methyltestosterone Residues in Aquatic Products
摘要
Abstract
A method to determine 17α-methyltestosterone (MET) residues in aquatic products was developed using LC-C18 solid phase extraction (SPE) combined with HPLC. Samples were extracted with diethyl ether under ultrasonic assistance. After concentration by rotary evaporation, the extract was defatted with n-hexane, cleaned up using an LC-C18 column, blown to dryness and dissolved again before reversed-phase HPLC analysis using a mobile phased made up of methanol and 0.1%formic acid (1:1, V/V ). The average recovery rates of the HPLC method for MET at spike concentrations of 7.0–200μg/kg were in the range of 82.0%–86.0%with a relative standard deviation (RSD) less than 7%. The limit of quantification (LOQ) for MET was 7.0μg/kg.关键词
甲基睾酮/高效液相色谱/固相萃取/水产品Key words
17α-methyltestosterone (MET)/high performance liquid chromatography (HPLC)/solid phase extraction (SPE)/aquatic products分类
轻工纺织引用本文复制引用
李佩佩,梅光明,张小军,陈雪昌,郭远明,龙举..固相萃取-高效液相色谱法测定水产品中的甲基睾酮残留量[J].食品科学,2012,33(22):282-285,4.基金项目
浙江省重点科技创新团队项目(2010R50028)；浙江省科技计划项目(20120F30021) （2010R50028）
