中国食品学报2012，Vol.12Issue(10)：76-81,6.
改性壳聚糖Cu(Ⅱ)配合物树脂对肌红蛋白的水解作用研究
Effect of Chemical Modified Chitosan Resin Complexed with Copper (Ⅱ) on Myoglobin Hydrolysis
摘要
Abstract
Chitosan and copper (II) ion were used as primary materials to prepare chemical modified chitosan resin complexed with copper ion (CMCR), which could be recycled. The hydrolyzing ability of CMCR was investigated through Ninhydrin Colorimetric method and SDS-PAGE electrophoresis method. The results demonstrated that myoglobin can be hydrolyzed more than 80% at physiological pH, 60 ℃ for 24 hours Copper ions ehelated on the resin hold a desirable stability, more than 98.17% copper ions still complexed with the resin after the first reaction. CMCR could be reused for 4 times while the catalytic ability maintains 85% of the original. The results conformed that CMCR is a very promising medium for hydrolyzing protein in the area of food etc.关键词
金属离子Cu(Ⅱ)/壳聚糖/树脂/肌红蛋白/催化水解Key words
copper(II)/ chitosan/ resin/ myoglobin/ hydrolysis引用本文复制引用
丁湄,梁鹏,王星宇,汪东风,张莉..改性壳聚糖Cu(Ⅱ)配合物树脂对肌红蛋白的水解作用研究[J].中国食品学报,2012,12(10):76-81,6.基金项目
海洋公益性行业科研专项(201005020-6) （201005020-6）
国家自然科学基金项目(20807040) （20807040）
国际合作项目(2010DFA31330) （2010DFA31330）
