现代食品科技2013，Vol.29Issue(1)：167-169,180,4.
桑叶中1-脱氧野尻霉素分离纯化的工艺研究
Isolation and purification of 1-deoxynojirimycin from Morus alba L.leaves
罗蕾蕾 1吴俊1
作者信息
- 1. 湖北武汉军事经济学院军需系给养勤务教研室,湖北武汉430035
- 折叠
摘要
Abstract
This paper studied the technology for isolation and purification of 1-Deoxynojirimycin (DNJ) from Morus alba L. leaves by using AB-8 macroporous resin and 732 H type cation exchange resin sequentially, with the content and recovery rate of DNJ as indexes. The result showed that AB-8 resin was suitable for the preliminary isolation and purification of DNJ. At pH 4.0, the maximum loaded amount of Morus alba L. leaves extract was 2BV and the recovery rate of DNJ was 98% by collecting the solution flowing out at lBV/h as flowing rate. The content of DNJ was enhanced to 4.9%. 732H type cation exchange resin chromatography was used to purify continuously. Elution order is: 1 BV H2O-1.5 BV 0.3 M NH3-2BV 0.5 M NH3. The final output was obtained by collecting the fraction with pH 9.0-10.0. The final total DNJ content was 16.7%. The technology is suitable for large scale manufactory of extract rich of DNJ.关键词
桑叶/1-脱氧野尻霉素/大孔树脂/阳离子树脂/纯化Key words
Morus alba L. leaves/1-Deoxynojirimycin/macroporous resin/cation exchange resin/purification引用本文复制引用
罗蕾蕾,吴俊..桑叶中1-脱氧野尻霉素分离纯化的工艺研究[J].现代食品科技,2013,29(1):167-169,180,4.
