食品工业科技2013，Vol.34Issue(1)：125-128,4.
大鲵胃蛋白酶分离纯化及其性质的研究
Purification and characterization of pepsin from stomach of Andrias davidianus
摘要
Abstract
With A davidianus pepsin as raw materials, extraction and purification of pepsin from stomach of Adavidianus were studied. Pepsin was isolated by extraction with PBS buffer, precipitation with 80% saturation ammonium sulfate,ion-exchange on DEAE-52 cellulose,gel filtration on Sephadex G-100 and HPLC.Finally the gotten pepsin was purified 239.87 times.The recovery rate was 4.4%.The pepsin was estimated to be 31 ku with SDS-PAGE. The optimal temperature for pepsin activity was 40℃,lower than 40℃ the pepsin was stable, when the temperature raised,the activity decreased rapidly.The optimum pH was 2,the activity was stable in the pH range of 2~6.Further studies showed that the activity was rapidly decreased by EDTA,BrAc,NBS.Using casein as substrate,under the pH2.0,40℃,Km was 7.3×103mg/L,Vmax was 2.674μg/min.关键词
大鲵/胃蛋白酶/纯化/性质Key words
Andrias davidianus/ pepsin/ purification/characterization分类
轻工纺织引用本文复制引用
成芳,闫欣,李伟,曲敏,佟长青,金桥..大鲵胃蛋白酶分离纯化及其性质的研究[J].食品工业科技,2013,34(1):125-128,4.基金项目
海洋局公益性行业科研专项(201205022-7、201005024-3) （201205022-7、201005024-3）
辽宁省高等学校优秀人才支持计划(2009R13) （2009R13）
湖南省科学计划厅科技计划项目(2010FJ6096) （2010FJ6096）
张家界市科技局科技计划项目(2010ZD019). （2010ZD019）
