食品工业科技2013，Vol.34Issue(1)：137-139,144,4.
离子交换法纯化罗非鱼血超氧化物歧化酶的研究
Research on purification conditions of superoxide dismutase from tilapia blood by ion exchange chromatography
摘要
Abstract
To get a reasonable,simple and efficient purification process of superoxide dismutase( SOD) from tilapia blood,the purification conditions of SOD by ion exchange chromatography( IEX)were explored and optimized.At last,the optimized purification conditions of SOD were fixed as follows;the mobile phase was pH8.0 20mmol/L Tris -HCI buffer,the stepwise elution method was 10%B、20min→30%B、15min→100% B、15min,the optimum sample volume was 4mL and the maximum flow rate was 150cm/h.The results showed that the specific enzyme activity of the purified tilapia blood SOD was 4052U/mg protein with a yield of 59.7% and a purification fold of 942 times, respectively. The optimized purification technology of SOD could provide a scientific and reliable technical guidance for future purification research and commercial process of SOD.关键词
罗非鱼血/超氧化物歧化酶(SOD)/离子交换(IEX)/优化Key words
tilapia blood/superoxide dismutase(SOD) /ion exchange chromatography( IEX)/optimization分类
轻工纺织引用本文复制引用
李来好,刘在军,岑剑伟,杨贤庆,郝淑贤,周婉君,陈胜军..离子交换法纯化罗非鱼血超氧化物歧化酶的研究[J].食品工业科技,2013,34(1):137-139,144,4.基金项目
国家现代农业产业技术体系项目(CARS-49) （CARS-49）
国家十二五科技支撑计划项目(2012BAD28B06) （2012BAD28B06）
国家农业科技成果转化资金项目(2010GB23260577,2010GB2E000335) （2010GB23260577,2010GB2E000335）
广东省科技计划项目(2009A020700004) （2009A020700004）
广西科学研究与技术开发计划项目(11107005-2) （11107005-2）
