食品工业科技2013，Vol.34Issue(1)：140-144,5.
响应面法优化一株中度嗜盐菌的培养条件
Optimization of culture conditions of a moderately halophilic bacteria by response surface methodology
摘要
Abstract
The culture conditions of a moderately halophilic bacteria strain screened from the surface of bacon were optimized by response surface methodology.Based on single factor tests,Plackett-Burman design was applied to screen key components in culture conditions.Then, the steepest ascent method was adopted to determine the maximum points.Finally,the concentrations of key components were confirmed by central composite deign and response surface methodology.It was showed that salinity,initial pH and culture temperature were key factors.The optimized medium consisted of 10g/L tryptone,5g/L yeast extract,54.8g/L NaCl,and the optimized condition consisted of initial pH7.9, culture temperature 32.8℃, liquid volume 70mL in 250mL flask, orbital shaker speed 120r/min. In optimized conditions,the bacteria density expressed by net added value of OD600nm was 3.349±0.021 after 2d,which was 13% higher than before.关键词
中度嗜盐菌/培养条件/响应面法/优化Key words
moderately halophilic bacteria/culture conditions/response surface methodology/optimization分类
轻工纺织引用本文复制引用
陈蔚青,李一飞,申屠超..响应面法优化一株中度嗜盐菌的培养条件[J].食品工业科技,2013,34(1):140-144,5.基金项目
杭州市科技发展计划项目(20110232B19). （20110232B19）
