食品工业科技2013，Vol.34Issue(1)：247-250,253,5.
二次正交旋转组合设计优化香菇废菌棒蛋白质的提取工艺
Optimization of extraction technology for protein in Lentinus edodes waste substrate by quadratic regression rotation-orthogonal combination design
摘要
Abstract
The quadratic regression rotation-orthogonal combination design was applied to optimize the extraction conditions of protein in Lentinus edodes waste substrate. On the basis of single-factor test, the mathematical regression model was established about the dependent variable (extraction yield of protein in Lentinus edodes waste substrates)and independent variables( alkali concentration,extraction temperature, extraction time, ratio of solvent to material) through quadratic regression rotation - orthogonal combination design and stat analysis. The results indicated that the obtained regression equation reached to significant level without any lack of fit factor.The optimum condition of protein extraction was alkali concentration of 0.11mol/L,72℃,104min extraction,47:1 of ratio of solvent to material,and the predictive maximum yield of protein in Lentinus edodes waste substrate was 2.38mg/g. The average validation value was 2.42mg/g. The relative error between them was 0.04mg/g. The optimized technology was stable,viable and feasible.关键词
香菇废菌棒/蛋白质/二次正交旋转组合设计Key words
Lentinus edodes waste substrate/ protein/quadratic regression rotation-orthogonal combination design分类
轻工纺织引用本文复制引用
陈海燕,刘青娥,钟仙龙,肖建中..二次正交旋转组合设计优化香菇废菌棒蛋白质的提取工艺[J].食品工业科技,2013,34(1):247-250,253,5.基金项目
浙江省自然科学基金项目(Y2111237),浙江省重点学科(生态学). （Y2111237）
