食品工业科技2013，Vol.34Issue(1)：309-311,316,4.
比色法测定不同产地黑豆皮中总黄酮的含量
Determination of total flavonoids in black soybean seed coat from different regions by colorimetric method
摘要
Abstract
Objective:To determine the flavonoids content in black soybean seed coat from eight different regions by colormetric method. Methods: Colormetric method was used to determine the total flavonoid contents in black soybeen seed coat from diferent regions at wavelength 510nm by taking rutin as standard substance, and Al( NO4)3 as chromogenic reagenLThe optimum extraction process of total flavonoids in black soybean seed coat was selected for ensure accuracy of the results.Results:The linear equation was Y = 12×-0.0009(R2 =0.9999) ,and the calibration curve was linear in the range of 0.003~0.057mg/mL.The average recovery was 104.6%.The contents of total flavonoids in black soybean seed coat from different regions were 6.873%~ 17.17%. Conclusion; The method was simple and rapid.The result showed that different origin of the content was quite different,with higher levels of Shanxi and Anhui.关键词
比色法/黑豆皮/总黄酮/含量测定Key words
Colorimetric method/black soybean seed coat/total flavonoids/the determination of content分类
轻工纺织引用本文复制引用
张翠,张丽娟,刘占云,刘岱琳..比色法测定不同产地黑豆皮中总黄酮的含量[J].食品工业科技,2013,34(1):309-311,316,4.基金项目
天津市科技支撑计划资助(09ZCKFNC01000). （09ZCKFNC01000）
