食品与机械2012，Vol.28Issue(6)：142-146,189,6.DOI:10.3969/j.issn.1003-5788.2012.06.035
乌饭树树叶多糖提取及纯化工艺优化研究
Study on the extraction and purification technology of polysaccharides from Vaccinium Bracteatum Thunb.leaves
摘要
Abstract
The optimal extraction process of polysaccharides from Vaccinium Bracteatum Thunb. Leaves (VBTL) was established by response surface method (RSM). The optimal extraction process was established by response surface analysis as following: pH 9, 5, temperature 55℃ , duration 2. 5 hour, and material /water ratio 1 : 45(m : V). By this optimized process 68. 629 1 mg polysaccharide could be obtained from one gram VBTL, the purity of VBTL poly-saccharides was 38. 27%. The orthogonal experiment was carried out to obtain the optimum conditions of the purification of VBTL poly-saccharides. The optimal conditions were as the following: the alcohol precipitation static time was 6 h, the volume ratio of extracting agent and alcohol was 1 : 5, the ethanol concentration (V : V) was 95%. By this process, the purity of polysaccharides increased from 33.27% to 52. 03%.关键词
乌饭树树叶/多糖/提取/纯化Key words
Vaccinium Bracteatum Thunb. leaves (VBTL)/ polysaccharides / extraction/ purification引用本文复制引用
程素娇,邓璐璐,章海燕,夏肖肖,张晖,钱海峰,齐希光,王立..乌饭树树叶多糖提取及纯化工艺优化研究[J].食品与机械,2012,28(6):142-146,189,6.基金项目
江苏省自然科学基金(编号:BK2010146) （编号:BK2010146）
生态纺织教育部重点实验室(江南大学)开放课题(编号:KLET0904) （江南大学）
江南大学自主科研青年基金项目(编号:JUSRP11016) （编号:JUSRP11016）
