食品与机械2013，Vol.29Issue(1)：131-134,4.DOI:10.3969/j.issn.1003-5788.2013.01.032
大孔吸附树脂纯化玉竹总黄酮工艺研究
Purification technology of total flavonods from Rhizoma Polygonati Odorati with macroporous resin methods
钟方丽 1王晓林 1王志敏 1陈帅1
作者信息
- 1. 吉林化工学院化学与制药工程学院,吉林吉林132022
- 折叠
摘要
Abstract
The type of macroporous resins were screened and the optimum purification conditions of total flavonods in Rhizoma Polygonati Odorati. were determined according to static state adsorption and desorption tests. On the base of single factor experimental results, the purification technology of total flavonods with D-101 macroporous resin was studied applying five-factor and four-level orthogonal test. The optimum purification conditions were obtained using adsorption and desorption rate as evaluating indicators. Total flavonods in Rhizoma Polygonati Odorati could be purified well with D-101 macroporous resin. On condition that the pH value of Rhizoma Polygonati Odorati, extraction solution, adsorption time, ratio of ethanol (desorption solution) volume to the resin weight and desorption time were 8, 5 h, 25 times, 60% , 3. 5 h respectively. The purity of crude total flavonods could be changed from 4 mg./g to a-bout 21 mg/g under the optimum purification conditions. The experimental results have shown that D-101 macroporous resin had better ability for purifying total flavonods in Rkizoma Polygonati Odorati. by which the total flavonods content was above 5 times higher than
the raw material. The purification method obtained in the paper is easy to operate, safe and cheap, which has comparatively higher practical application value.关键词
玉竹/总黄酮/大孔树脂/纯化/吸附/解吸附Key words
Rhizoma Polygonati Odorati. / total flavonods/ macroporous resin/ purification/ adsorption/ desorption引用本文复制引用
钟方丽,王晓林,王志敏,陈帅..大孔吸附树脂纯化玉竹总黄酮工艺研究[J].食品与机械,2013,29(1):131-134,4.
