中国食品添加剂Issue(6)：93-97,5.
紫外诱变球毛壳霉原生质体选育木聚糖酶高产菌株
Breeding of high yield xylanase chaetomium globosum protoplast by ultraviolet mutation
摘要
Abstract
[ Objective ] High yield xylanase strains were found by screening with ultraviolet mutagenesis of Chaetoraium globosum protoplast. [ Methods ] Chaetomium globosum AS3. 3601 protoplasts were prepared under different conditions. The effect of different parameters such as enzyme concentration, temperature and time on protoplast production and regeneration rate was analyzed. The dose of UV mutagenesis was determined by single factor experiments. Xylanase activity was measured by DNS methods after initial screening and rescreening. [ Results ] Judged by combined conditions considering production rate and regeneration rate of protoplasm, the best conditions for preparing protoplast was at enzyme concentration of 1. 5% , reaction temperature of 30℃ , and time of 4h. The protoplasts treated with 60s of UV irradiation show a xylanase activity of 9980 IU /mL. [Conclusion] xylanase activity of YZ -8 with mutation strain increased 92. 6% compared with the original strain and it is genetically stability.关键词
球毛壳霉/木聚糖酶/原生质体/紫外Key words
chaetomium globosum/ xylanase/ protoplast/ ultraviolet分类
轻工纺织引用本文复制引用
杨健,姚笛,王颖,张丽媛,钱丽丽..紫外诱变球毛壳霉原生质体选育木聚糖酶高产菌株[J].中国食品添加剂,2012,(6):93-97,5.基金项目
黑龙江省教育厅科学技术研究项目(11551324). （11551324）
